Fig. 5
Immunofluorescence and western blot analysis of CORO1C and SV2B within HA-bs and MB cells, alongside CORO1C IF analysis within GB cells. (a) IF staining revealed that CORO1C, SV2B, and β-actin exhibited uniform staining across normal human astrocytes, with CORO1C showing nuclear localisation and SV2B showing cytoplasmic localisation. (b) IF staining within DAOY MB cells showed an increased fluorescence of SV2B in MB cells when compared to HA-bs cells, alongside a nucellar localisation of CORO1C similar to the one observed in HA-bs cells. (c) IF staining within GB cells revealed that there was increased fluorescence of CORO1C within both, U251MG and U87MG cells in comparison to HA-bs cells. (d) Chemiluminescent images showing the expression of β-actin, CORO1C, and SV2B within HA-bs, U251MG, U87MG, and DAOY cells. (e) CORO1C protein expression was significantly upregulated within all GB and MB cell lines in comparison to normal HA-bs cells (U251MG, p < 0.0001; U87MG, p < 0.003; DAOY, p < 0.0002). (f) SV2B protein expression was significantly upregulated within U251MG, U87MG, and DAOY cells in comparison to HA-bs cells (p < 0.037, p < 0.003, p < 0.0001, respectively). (g) β-actin protein expression revealed no significant difference between the normal astrocytes and GB and MB cells. Results are based on three independent experiments, n = 3. All statistical analyses were conducted using ordinary one-way ANOVA, followed by Dunnett’s multiple comparisons test to determine the significant differences in expression between normal human astrocytes, GB and MB cells. Error bars represent the SD of the mean. Original blots/gels are presented in Supplementary Fig. 1.ns non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
