Fig. 6

Immunohistochemistry analyses of CORO1C. (a) All age groups showed significantly increased levels of CORO1C in comparison to their normal counterparts. The age groups of 0–20 years showed the most significantly increased levels of CORO1C in comparison to normal and normal adjacent tissues controls (Normal Tissue versus Age 0–20, p < 0.0001; Adjacent brain tissue versus Age 0–20, p < 0.0001; Normal Tissue versus Age 21–40, p < 0.0009; Adjacent Brain Tissue versus Age 21–40, p < 0.0001; Normal Tissue versus Age 41–60, p < 0.0019; Adjacent Brain Tissue versus Age 41–60, p < 0.0001; Normal Tissue versus Age 61–80, p < 0.0360; Adjacent Brain Tissue versus Age 61–80, p < 0.0012). (b) Significantly higher levels of the CORO1C protein were observed within MB and GB, in comparison to the rest of the malignancies and their normal counterparts (Adjacent Brain Tissue versus Astrocytoma, p < 0.0014; Normal Tissue versus Ependymoma, p < 0.0027; Adjacent Brain Tissue versus Ependymoma, p < 0.0001; Adjacent Brain Tissue versus Oligodendroglioma, p < 0.0037; Normal Tissue versus MB, p < 0.0001; Adjacent Brain Tissue versus MB, p < 0.0001; Normal Tissue versus GB, p < 0.0001; Adjacent Tissue versus GB, p < 0.0001). (c) Significant differences between tumour grades 2, 3 and 4 in comparison to normal brain tissue were observed. Grade 1 tumours showed significantly higher expression in comparison to normal adjacent tissue. Significant differences between grades 3 and 4 and grades 1 and 2 (Adjacent Brain Tissue versus Grade 1, p < 0.0460; Normal Tissue versus Grade 2, p < 0.0395; Adjacent Brain Tissue versus Grade 2, p < 0.0009; Normal Tissue versus Grade 3, p < 0.0003; Adjacent Brain Tissue versus Grade 3, p < 0.0001; Normal Tissue versus Grade 4, p < 0.0001; Adjacent Brain Tissue versus Grade 4, p < 0.0001; Grade 1 versus Grade 3, p < 0.0012; Grade 1 versus Grade 4, p < 0.0001; Grade 2 versus Grade 4, p < 0.0001). (d) Microscopic images of CORO1C stained tissues sections illustrating the staining intensity within normal brain tissue, tumour adjacent tissue, MB and GB tissues at × 100 magnification. (e) Microscopic images of CORO1C stained tissues cores of normal brain tissue, tumour adjacent tissue, and different brain malignancies at × 40 magnification. Results are based on three independent scoring assessments, n = 3. All statistical analyses were conducted using ordinary one-way ANOVA, followed by Dunnett’s multiple comparisons test to determine the significant differences in CORO1C expression. Error bars represent the SD of the mean.ns non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NT normal tissue, NAT normal adjacent tissue, AST astrocytoma, MB medulloblastoma, GB glioblastoma.