Fig. 7
Transient transfections of MB cells. (a, b) miR-206 and miR-383 were significantly upregulated after transfections with miR-206 and miR-383 mimics (miR-206, p < 0.0001; miR-383, p < 0.0001). (c) CORO1C was significantly downregulated post transfection with miR-206 mimic (p < 0.0009). (d) SV2B was significantly downregulated post transfection with miR-383 mimic (p < 0004). (e, f) MB cell viability was significantly decreased post transfection with miR-206 and miR-383 mimics (miR-206, p < 0.0032; miR-383, p < 0.009). (g, h) MB cells lost their colony forming abilities post transfections with miR-206 and miR-383 mimics. (i) Microscopic images of CF within MB cells post exposure to miR-206 and miR-383 mimics (× 40 magnification). (j) Western blotting analysis showed that the protein levels of CORO1C were significantly decreased in comparison to the negative control condition post exposure to miR-206 mimic (p < 0.0001). The protein levels of SV2B were also significantly reduced in comparison to the negative control condition post exposure to miR-383 mimic (p < 0.0009). (k) Chemiluminescent images showing the expression of β-actin, CORO1C, and SV2B within negative control miRNA #1, positive control miR-1, and miR-206 and miR-383 mimics. Results are based on three independent experiments, n = 3. All statistical analyses were conducted using ordinary one-way ANOVA, followed by Dunnett’s multiple comparisons test. Error bars represent the SD of the mean. Original blots/gels are presented in Supplementary Fig. 1. ns non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
