Fig. 8

Transient transfections of GB cells. (a, b) miR-206 and miR-383 were significantly upregulated after transfections with miR-206 and miR-383 mimics within both GB cell lines (U251MG miR-206, p < 0.0001; U87MG miR-206, p < 0.0034; U251MG and U87MG miR-383, p < 0.0001). (c) CORO1C was significantly downregulated post transfection with miR-206 mimic in U251MG and U87MG cells (U251MG, p < 0.0001; U87MG, P < 0.0001). (d) SV2B was significantly downregulated post transfection with miR-383 mimic with both GB cell lines (U251MG, p < 0.0001; U87MG, p < 0.0001). (e, f) GB cell viability was significantly decreased post transfection with miR-206 and miR-383 mimics in both, U251MG and U87MG cells (U251MG miR-206, p < 0.0039; U87MG miR-206, p < 0.04; U251MG miR-383, p < 0.002; U87MG miR-383, p < 0.001). (g, h) GB cells lost their colony forming abilities post transfections with miR-206 and miR-383 mimics (p < 0.0001). (i) Microscopic images of CF within GB cells post exposure to miR-206 and miR-383 mimics (× 40 magnification). (j) Western blotting analysis in U251MG showed that the protein levels of CORO1C were significantly decreased in comparison to the negative control condition post exposure to miR-206 mimic (p < 0.0001), whilst the levels of CORO1C were unaffected in the miR-383 mimic condition. The protein levels of SV2B were also significantly reduced in comparison to the negative control condition post exposure to miR-383 mimic (p < 0.0001). Additionally, the levels of SV2B were also downregulated in the miR-206 mimic condition (p < 0.0001). (k) Western blotting analysis in U87MG showed that the protein levels of CORO1C were significantly decreased in comparison to the negative control condition post exposure to miR-383 mimic p < 0.0002), whilst the levels of CORO1C were unaffected in the miR-206 mimic condition. The protein levels of SV2B were also significantly reduced in comparison to the negative control condition post exposure to miR-383 mimic(p < 0.0001). Additionally, the levels of SV2B were not affected in the miR-206 mimic condition. (l, m) Chemiluminescent images showing the expression of β-actin, CORO1C, and SV2B within U251MG and U87MG cells post exposure to negative control miRNA #1, positive control miR-1, and miR-206 and miR-383 mimics. Results are based on three independent experiments, n = 3. All statistical analyses were conducted using ordinary one-way ANOVA, followed by Dunnett’s multiple comparisons test. Error bars represent the SD of the mean. Original blots/gels are presented in Supplementary Fig. 1. ns non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.