Fig. 4
From: LncRNA CTD-2555A7.2 promotes bone formation with LncRNA-specific cascade amplification strategy
miR-381-3p inhibited four target genes of Wnt signaling pathway. A, C Protein expression of APC, LEF1, LRP6 and wnt5a of hMSCs treated with mimic-381-3p (compared with mimic-NC), as detected by western blot (mean ± S.D., n = 3). B, D Protein expression of APC, LEF1, LRP6 and wnt5a of hMSCs treated with inhibitor-381-3p, as detected by western blot(mean± S.D., n > 3). E-H Binding effect of miR-381-3p and 3′UTR sequences of Apc, Lef1, Lrp6 and wnt5a, as detected by luciferase reporter assay (mean ± S.D., n = 3). Luc-vec: empty luciferase reporter plasmid. Luc-mut: luciferase reporter plasmid containing mutant 3′UTR. Luc-WT: luciferase reporter plasmid containing wild-type 3′UTR.*P < 0.05, **P < 0.01, ***P < 0.001
