Table 1 Primers sequences for qRT-PCR.
From: LncRNA CTD-2555A7.2 promotes bone formation with LncRNA-specific cascade amplification strategy
Target gene | Sequences (5’→3’) |
|---|---|
Human Alp-Forward | GGCCATTGGCACCTGCCTTA |
Human Alp-Reverse | ACCCATCCCATCTCCCAGGAA |
Human Ocn-Forward | GGTGCAGCCTTTGTGTCCAAGC |
Human Ocn-Reverse | GTCAGCCAACTCGTCACAGTCC |
Human Sp7-Forward | CCTCTGCGGGACTCAACAAC |
Human Sp7-Reverse | AGGGTGGGTAGTCATTTGCAT |
Human Gapdh-Forward | CATGGAGAAGGCTGGGGCTC |
Human Gapdh-Reverse | CACTGACACGTTGGCAGTGG |
Mouse Alp-Forward | GTTGCCAAGCTGGGAAGAACAC |
Mouse Alp-Reverse | CCCACCCCGCTATTCCAAAC |
Mouse Runx2-Forward | CGCCCCTCCCTGAACTCT |
Mouse Runx2-Reverse | TGCCTGCCTGGGATCTGTA |
Mouse Gapdh-Forward | TGCACCACCAACTGCTTAG |
Mouse Gapdh-Reverse | GGATGCAGGGATGATGTTC |
CTD-2555A7.2-Forward | TGCCTTCACCTCTGCTCTTC |
CTD-2555A7.2-Reverse | AGGGTGGGTTGAGGTAGAGG |
Human Tcf7-Forward | GCGGACATCAGCCAGAAG |
Human Tcf7-Reverse | TCACAGTATGGGGGAGCTGT |
Human Smad4-Forward | GCCGCATGAAGTGGATAAGG |
Human Smad4-Reverse | TCTGGTCCGCGTGTTCTTT |
hsa-miR-381-3p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAGAG |
hsa-miR-381-3p-Forward | CGCGTATACAAGGGCAAGCT |
hsa-miR-381-3p-Reverse | CCAGTGCAGGGTCCGAGGT |
hsa-miR-15b-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCCAT |
hsa-miR-15b-5p-Forward | TAGCAGCACAATAGTGT |
hsa-miR-15b-5p-Reverse | CCAGTGCAGGGTCCGAGGT |
hsa-miR-21-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAGAC |
hsa-miR-21-5p-Forward | TAGCTTATCAGACTGATG |
hsa-miR-21-5p-Reverse | CCAGTGCAGGGTCCGAGGT |
hsa-miR-146a-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCAGT |
hsa-miR-146a-5p-Forward | TGAGAACTGAATTCCAT |
hsa-miR-146a-5p-Reverse | CCAGTGCAGGGTCCGAGGT |
Mouse Hes1-Forward | CCAGCCAGTGTCAACACGA |
Mouse Hes1-Reverse | AATGCCGGGAGCTATCTTTCT |
Mouse Tcf7-Forward | CAGAATCCACAGATACAGCA |
Mouse Tcf7-Reverse | CAGCCTTTGAAATCTTCATC |
Mouse Smad4-Forward | TGCCTGGTCTGTGTGTTTGG |
Mouse Smad4-Reverse | GCCACGGAGTTGTCATAGGA |
Human Apc-Forward | TGACCTCCACTCGCCTGTCTTG |
Human Apc-Reverse | GCCTTCTCGTTAGCCCTGAATGTC |
Human Lef1-Forward | CTTTATCCAGGCTGGTCTGC |
Human Lef1-Reverse | TCGTTTTCCACCATGTTTCA |
Human Lrp6-Forward | GCTTCTGCGTGCTGCTGAGAG |
Human Lrp6-Reverse | CCTCCAAGCCTCCAACTACAATCG |
Human wnt5a-Forward | CAGGCGGTGGCAAGCAGAAC |
Human wnt5a-Reverse | AGTGTGGGCAGGCAGTGGTC |
LA16c-306A4.2-Forward | CAGGTGCTCAAGGTCTTCCT |
LA16c-306A4.2-Reverse | TGGCTGAGACTGCTGTGATT |
HP09025-Forward | GCTCCATGGAGACCTCAAGA |
HP09025-Reverse | CAGGCTGTGTGGTTTGCTTT |
TP73-AS1-Forward | GGGAAATACCCGACCTCAAC |
TP73-AS1-Reverse | TCCAGGTCCCAAGTTCTTCC |
AP001476.3–201-Forward | GCTGGGACTTCTGGACTGTG |
AP001476.3–201-Reverse | CCAGGGTCTGGTGTCTGTTC |
RP11-405M12.2-Forward | CACAGCCACCTCTTCCTCTC |
RP11-405M12.2-Reverse | TGCTGAGGAAGGTGAGGAAG |
