Fig. 2

LDH2 is expressed in male gametocytes and microgametes. (A) Schematic diagram of the ldh2 alleles in parental (wildtype) parasites and in the transgenic lines LDH2/GFP and LDH2-KO. The ldh2 gene is shown in grey with coding sequence (wide bars) and 5′ and 3′ untranslated regions (narrow bars). Also shown are the gfp module, the selectable marker (hdhfr), and positions of primers P1-P3 used for diagnostic PCR amplification. (B) PCR with primers P1 and P3 diagnostic for integration of the modified ldh2 alleles into the ldh2 locus, or with primers P1 and P2 diagnostic for absence of the unmodified parental ldh2 allele. See Materials and Methods section for primer sequences. Corresponding uncropped DNA agarose gels are shown in Supplementary Fig. S3. (C) Live fluorescence confocal images of exflagellating LDH2/GFP and LDH2-KO microgametocytes with emerging microgametes (arrows). Scale bar 5 μm.