Fig. 2

Model Graphics obtained in Design-Expert software (v.11.1.2.0): 3D surface plots of biomass productivity (g L−1 d−1) and growth rate (d−1) predicted for mutant 8G (a), 7Y (b) and 31 W (c) of C. vulgaris within the range of the nutrient concentration tested in the optimization trial and the interaction between those 2 factors, considering the average value of the remaining factors tested. The black arrows indicate the conditions in which the highest values of that response would be obtained according to that model. Cooler colors (blue-green) indicate suboptimal conditions, yellow represents intermediate values and warmer colors (orange-red) indicate optimal conditions for that response.