Fig. 5

Model Graphics obtained in Design-Expert software (v.11.1.2.0): 3D-surface plots of color: L* (brightness/darkness coordinate) predicted for mutant 8G, b* (yellow/blue coordinate) predicted for mutant 7Y and a* (green/red coordinate) predicted for mutant 31 W of C. vulgaris within the range of the nutrient concentration tested in the optimization trial and the interaction between those 2 factors, considering the average value of the remaining factors tested. Cooler colors (blue-green) indicate lower values, yellow represents intermediate values and warmer colors (orange-red) indicate higher values. The darker green color aimed for mutant 8G is represented by the cooler colors, corresponding to lower L* values (darker), a stronger yellow color is represented by warmer colors for mutant 7Y, corresponding to higher b* values (more yellow) and a whiter tone in the case of mutant 31 W is also represented by the warmer colors, corresponding to higher a* values (less green).