Table 3 Factors and levels used in the macro- and micronutrient screening assays for each mutant strain of C. vulgaris (8G, 7Y, and 31 W).
Factor | Levels | |||||||
|---|---|---|---|---|---|---|---|---|
Strain | 8G | 7Y | 31 W | |||||
Macronutrient screening | ||||||||
[N] (mM) | 20.0 | 80.0 | 20.0 | 80.0 | 20.0 | 80.0 | ||
[P] (mM) | 20.0 | 80.0 | 20.0 | 80.0 | 20.0 | 80.0 | ||
[Ca] (mM) | 0.50 | 4.00 | 0.40 | 2.00 | 0.40 | 2.00 | ||
[Na]/[K] | 0.30 | 0.90 | 0.30 | 0.90 | 0.30 | 0.90 | ||
[Mg] (mM) | 3.00 | 12.0 | 3.00 | 12.0 | 3.00 | 12.0 | ||
Nitrogen source | Ammonium | Urea | Ammonium | Urea | Ammonium | Nitrate | ||
Micronutrient screening | ||||||||
[Cu] (mM) | 0.0020 | 0.2000 | 0.0060 | 0.0600 | 0.0060 | 0.0600 | ||
[B] (mM) | 0.0500 | 0.5000 | 0.0500 | 0.5000 | 0.0500 | 0.5000 | ||
[Zn] (mM) | 0.0300 | 0.3000 | 0.0300 | 0.3000 | 0.0300 | 0.3000 | ||
[Mn] (mM) | 0.0200 | 0.2000 | 0.0200 | 0.2000 | 0.0200 | 0.2000 | ||
[Mo] (mM) | 0.0005 | 0.5000 | 0.0050 | 0.5000 | 0.0050 | 0.5000 | ||
[Ni] (mM) | 0.0001 | 0.0010 | 0.0001 | 0.0010 | 0.0001 | 0.0010 | ||
[Fe] (mM) | 0.0100 | 1.0000 | 0.0500 | 0.5000 | 0.0500 | 0.5000 | ||
[Vitamin solution] | 0.2500× | 2.5000× | 0.2500× | 2.5000× | 0.2500× | 2.5000× | ||
