Fig. 8 | Scientific Reports

Fig. 8

From: Potentiation of immune checkpoint blockade with an ITPP radiosensitizer studied with oxygen saturation measurements from photoacoustic imaging

Fig. 8

%sO2 after the first ITPP treatment correlated with intratumoral frequencies of Teffs following ITPP and ICB treatment. (a,b) The study timelines. PAI was performed 3 h after administering ITPP. ICB was administered immediately after PAI on the initial treatment day. ICB was administered 3 h after ITPP on days without PAI. Negative correlations between %sO2 three hours after ITPP and the frequencies of (c) CD8 and CD4 + FoxP3- T cells in CT26 tumors, (d) CD8 and CD4 + FoxP3- 4T1 tumors, (e) TCF-1 + CD8 and TCF-1 + CD4 + FoxP3- T cells in CT26 tumors, and (f) TCF-1 + cD8 and TCF-1 + CD4 + FoxP3- T cells in 4T1 tumors after two or three commination ITPP, αCTLA-4 and αPD-1 treatments. Positive correlations between %sO2 three hours after ITPP and the frequencies of TOX + CD8 and TOX + CD4 + FoxP3- T cells in (g) CT26 and (h) 4T1 tumors after ITPP, αCTLA-4 and αPD-1 treatments. Dotted lines represent the 95% confidence interval while the solid lines represent the linear best fit line. n = 11–12.

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