Fig. 1
Phylogenetic and conserved domain analysis of the CSRPs from the representative species of Phyla Mollusca, Arthropoda, and Chordata. The CSRPs were identified from cnidarias (Allacma fusca [Afu]), nemerteans (Lineus longissimus [Ll]), molluscans (Haliotis rufescens [Hr], Batillaria attramentaria [Ba], Crassostrea giga [Cg], Crassostrea sikamea [Cs], Mytilus edulis [Me], Azumapecten farreri [Afa], Mizuhopecten yessoensis [My], Argopecten irradians [Ai], Mytilus galloprovincialis [Mg]), arthropods (Folsomia candida [Fc], Eciton burchellii [Eb], Eufriesea Mexicana [Em], Vespa mandarinia [Vm], Polistes canadensis [Pc]), and chordates (Danio rerio [Dr], Sinocyclocheilus rhinocerous [Sr], Heptranchias perlo [Hp], Branchiostoma lanceolatum [Bl]). Afu (Cnidaria-hydra), Ll (Nemertea-bootlace worm), Hr (Mollusca-gastropod), Ba (Mollusca-gastropod), Cg (Mollusca, Bivalvia-scallop), Cs (Mollusca, Bivalvia-scallop), Me (Mollusca, Bivalvia-scallop), Afa (Mollusca, Bivalvia-scallop), My (Mollusca, Bivalvia-scallop), Ai (Mollusca, Bivalvia-scallop), Mg (Mollusca, Bivalvia-scallop), Fc (Arthropod), Eb (arthropod-ant), Em (arthropod-bee), Vm (arthropod-hornet), Pc (arthropod-wasp), Dr (Chordata/Vertebrata-finfish), Sr (Chordata/Vertebrata-finfish), Hp (Chordata/Vertebrata-shark), Bl (Chordata/Cephalocordata-amphioxus). Three CSRP homologous genes were screened out from both C. sikamea and C. gigas, and one CSRP homologous gene was selected from each of the remaining species. Values on the evolutionary branches represent bootstrap values. Gray shading indicates the presence of signal peptides, white indicates the absence of signal peptides, and red-highlighted fonts represent CSRP homologous proteins in C. sikamea and C. gigas. “I” to “V” represented the five clades. The “i”, “ii”, “iii”, and “iv” in the domain section were the numeric of conserved Cu/Zn-SOD domains. The rest of the shapes represent the domain superfamily. The MDR superfamily is a zinc-dependent alcohol dehydrogenase-like family, and the VSP superfamily is a Giardia variant-specific surface protein.
