Fig. 2 | Scientific Reports

Fig. 2

From: ADCY4 inhibits cAMP-induced growth of breast cancer by inactivating FAK/AKT and ERK signaling but is frequently silenced by DNA methylation

Fig. 2

Identification of ADCY4 silenced by promoter methylation in BC. (A) The CpG islands in the LTBP4 nucleotide sequence (EMBOSS-cpgplot). Each red arrows represents a single CpG island. (B) ADCY4 methylation in primary BC tissues (n = 171) and normal tissues (n = 11) measured by MSP. m-, methylated, u-, unmethylated. Gel images showed the representational graphs. (C) The differences in methylation levels of ADCY4 in primary BC (n = 793) and normal tissues (n = 97) from TCGA datasets. (D) The correlation between ADCY4 methylation and expression from TCGA-BRCA datasets was analyzed using cBioPortal online software (n = 1253, r=-0.19, p = 1.35E-11, < 0.001); (E) ADCY4 mRNA expression and methylation status in BC cell lines. m-, methylated, u-, unmethylated. (F) ADCY4 methylation status with 5-aza-2-deoxycytidine (A) and trichostatin A (T) treatments in BC cell lines. m-, methylated, u-, unmethylated. (G) ADCY4 expression with 5-aza-2-deoxycytidine (A) and trichostatin A (T) treatments in BC cell lines by qRT-PCR. **p < 0.01.

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