Fig. 5

Phosphorylation of AKT and ERK signaling is suppressed by ADCY4 ectopic expression. (A) MDA-MB-231 and MCF-7 cells with ADCY4 ectopic expression or control cells were treated with vehicle (DMSO) or SC79, WB analysis for phospho-AKT (Ser473) and AKT. β-actin was used as a loading control. (B) MDA-MB-231 and MCF-7 cells with ADCY4 ectopic expression or control cells were treated the same as in (A), the cell viabilities were measured by MTT assay at 24, 48, 72, and 96 h. (C) MDA-MB-231 and MCF-7 cells with ADCY4 ectopic expression or control cells were treated the same as in (A), the percentages of apoptotic cells were measured. (D) MDA-MB-231 and MCF-7 cells with ADCY4 ectopic expression or control cells were treated with vehicle (DMSO) or tBHQ, WB analysis for phospho-ERK1/2 (Thr202/Tyr204) and ERK. β-actin was used as a loading control. (E) MDA-MB-231 and MCF-7 cells with ADCY4 ectopic expression or control cells were treated the same as in (D), the cell viabilities were measured by MTT assay at 24, 48, 72, and 96 h. (F) MDA-MB-231 and MCF-7 cells with ADCY4 ectopic expression or control cells were treated the same as in (D), the percentages of apoptotic cells were measured. ***p < 0.001, **p < 0.01, *p < 0.05.