Fig. 5
Measurement of (A) xylanase and (B) CMCase activities of the secretomes of acidotolerant and acidophilic fungi grown on complex substrates (1.5 g/100 mL each of malt residue, sugarcane bagasse, and rice bran). All activities were measured at 50 °C. In each panel is shown the maximum specific activity (U/mg); the relative activity level at pH 1, 3, 5 and 7, scaled to the highest activity observed as 100%; and lastly the residual activity after heating at 70 °C for 20 min followed by overnight cooling at 4 °C before measuring activity at 50 °C. Each value is the average of duplicate measurements. Blanks containing no substrate were subtracted. On the left is shown an unscaled UPGMA (unweighted pair group method with arithmetic mean) tree.
