Fig. 2
TRIM22 promotes melanoma cell proliferation in vitro. (A) Western blots and qRT-PCR were performed to characterize the expression of TRIM22 in the control and TRIM22-overexpression A375 cells. (B) Western blots and qRT-PCR were performed to characterize the expression of TRIM22 in the control and TRIM22-knockdown A375 cells. (C–E) CCK-8 (C), colony formation (D) and colony growth proliferation (E) assays were performed to evaluate the growth ability of A375 cells with or without TRIM22 overexpression. (F–H) CCK-8 (C), colony formation (D) and colony growth proliferation (E) assays were performed to evaluate the growth ability of A375 cells with or without TRIM22 konckdown. (I–J) EdU assays were performed to evaluate the growth ability of the control, TRIM22-overexpression, and TRIM22-knockdown A375 cells. Scale bar, 200 μm. Quantitative analysis was performed using ImageJ software. All data were expressed as the mean ± SD. The standard deviations of independent experiments were represented by error bars. The data were analyzed by two-tailed unpaired t-test or One-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
