Fig. 6 | Scientific Reports

Fig. 6

From: E3 ligase TRIM22 promotes melanoma proliferation by regulating cell cycle progression through K63-linked ubiquitination of p21

Fig. 6

p21 mediates the melanoma proliferation effects of TRIM22. (A) Western blots were performed to characterize the expression of TRIM22 and p21 in the shControl, shp21, and shp21 + TRIM22 A375 cells. (B–C) CCK-8 assays were performed to evaluate the growth ability of shControl, shp21, shp21 + TRIM22 A375 cells. (D–E) Colony formation and colony growth proliferation assays were performed to evaluate the growth ability of shControl, shp21, shp21 + TRIM22 A375 cells. (F–G) EdU assays were performed to evaluate the growth ability of the control, shControl, shp21, shp21 + TRIM22 A375 cells. Scale bar, 200 μm. Quantitative analysis was performed using ImageJ software. All data were expressed as the mean ± SD. The standard deviations of independent experiments were represented by error bars. The data were analyzed by two-tailed unpaired t-test or One-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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