Fig. 2 | Scientific Reports

Fig. 2

From: Hinokiflavone is a novel CK2 inhibitor promoting apoptosis and synergizing with chemotherapeutic agents in cisplatin resistant bladder cancer cells

Fig. 2

Hinokiflavone promotes cell cycle accumulation at the sub-G1 phase and induces apoptosis in N/P(14) cells. (a) N/P(14) cells were treated with different concentrations of hinokiflavone for 24–48 h. Cell cycle distribution was analyzed by propidium iodide staining and flow cytometry. (b) Quantitative data of histograms are expressed as mean±S.D. (n = 2). (c) N/P(14) cells were treated with varying concentrations of hinokiflavone for 48 h, stained with Annexin V/7-AAD, and analyzed via flow cytometry. (d). Quantification of apoptotic cells (early- and late-stage) are expressed as mean±S.D. (n = 3). Statistical analyses were performed by unpaired two-tailed Student’s t-test. ***p < 0.001 compared with the control group.

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