Table 3 Oligonucleotides used in this study.
From: Dissecting the contribution of Kup and KimA to Enterococcus faecalis potassium homeostasis
Primers | Sequencea | Used for |
|---|---|---|
Kim-RT | GGATACGCATGAATAATTTGTCG | Determination of transcription start site of kimA promotor - reverse transcription |
Kim-1 Specific primer | AATTGGCAATGAATACCAAATC | Determination of transcription start site of kimA promotor - specific primer |
QT_RACE | CCAGT GAGCA GAGTG ACGAG GACTCGAGCTCAAGCTTTTTTTTTTTTTTTTT | Determination of transcription start site of kimA promotor - primer PolyT |
Qo_RACE | CCAGTGAGCAGAGTGACG | Determination of transcription start site of kimA promotor - specific primer PolyT |
KimA-F | CATGGATCCCCAGTGAATTTGTATGACC | Amplification of different upstream segments of the kimA gene and cloning in pTLGR plasmid |
KimA-R | CAAGGATCCGTCGTTTTAAATCCGCC | |
RevGFP | CCGCTCGAGCCTATTTGTATAGTTCATCC | Verification of insertion and orientation into pTLGR plasmid |
kimA-r | CGAAAGAGATAAAGGCGCCG | RT-qPCR for kimA gene |
kimA-l | TGCTTTGTCTCAATCGGGGA | |
kup-r | ATGTTGCTGTGGGAATGGCA | RT-qPCR for kup gene |
kup-l | CCAACGACACTTGTTCCGC |
