Fig. 1
The workflow for strand-specific RNA sequencing of in vitro cultured TPA for deep transcriptome sequencing. One million bacterial cells from an in vivo culture were inoculated in TPCM-2 media both in the presence or absence of Sf1Ep cells, and were incubated in low oxygen incubator (1.5% of oxygen). After 7 days, the RNA from both samples was extracted, and prior to library preparation, were treated as indicated. Multiple different treatments were performed before library preparation to ensure the transcripts would not be compromised. Directional RNA library preparation was performed, and each library was sequenced using Illumina NovaSeq to generate 30 Gbp of data per sample (100 million PE150 reads). Created with BioRender.com.
