Fig. 3
From: Multiplex detection of seven transgenes for human gene doping analysis
LOD results of the GD panel summarized as heatmaps. The GD panel was tested on two different serial dilutions of pooled transgenic plasmids with increasing amount of gDNA background. Each assay was tested in 12 replicates with the one-step (1 × PCR) and two-step (2 × PCR)-PCR protocol. GH_3, IGF1_1_1 and IGF1_1_2 were tested in 24 replicates as they were analyzed on two different isoforms. Green = LOD95, light green = LOD90, yellow = LOD80, grey = below LOD80.
