Fig. 6
From: Multiplex detection of seven transgenes for human gene doping analysis
Performance testing of the RM with the GD panel. Five to 80 cp of the RM were spiked into the iPLEX® reaction with increasing amounts of gDNA and tested with the two-step PCR protocol. Each input concentration was tested in 12 replicates. The assays GH_1, GH_2, IGF1_1_2, IGF1_3, VEGFD_2 and the GAPDH assay are not included on the RM and are therefore marked in grey. The results are summarized as heatmaps. Green = all replicates, light yellow = more than half of the replicates were called positive.
