Fig. 7
From: Multiplex detection of seven transgenes for human gene doping analysis
Degradation of the IGF1_2 extension primer (EP) in the panel version v1.0 and stabilization of the assay in the panel version v.1.1. Shown are exemplary spectra for the analysis of the RM (80 cp) with the panel version v1.0 (upper spectra) and v.1.1. (lower spectra). Upper spectra: Degradation of the IGF1_2 EP leads to an erroneous peak (highlighted with a red bar) in the spectrum of the IGF1_1_1 assay. IGF1_1_1 MUT calling by the TYPER software is disturbed by the unexpected EP-degradation peak (grey bar, upper left spectrum). Lower spectra: Upon PTO-stabilization of the IGF1_2 assay in the panel version v1.1, no degradation of the IGF1_2 EP and thus no erroneous peak in the IGF1_1_1 spectrum is seen anymore. Called MUT assays are highlighted with a blue line and bar. (Peaks marked with grayed-out dotted lines correspond to the MUT-extended EPO_1 assay (IGF1_1_1 spectra), the UEP of the GH_2 assay (IGF1_2 upper spectrum) and the MUT-extended VEGFD_1 assay (IGF1_2 lower spectrum)).
