Table 6 Hematological and biochemical responses of nile tilapia fingerlings fed commercial bile acid supplementation.

From: Impacts of commercial bile acids on growth performance, immune responses and expression genes of lipid metabolism in Nile tilapia fingerlings Oreochromis niloticus

Diet¹		D1	D2	D3	D4	D5	D6
Differential leucocytic counts Parameters	Lymphocytes	55.18^f ± 0.43	63.91^d ± 0.57	72.44^a ± 0.29	69.45^b ± 0.67	66.33^cd ± 0.55	61.11^e ± 0.44
	Monocytes	2.65^a ± 0.04	2.08^a ± 0.04	1.89^b ± 0.03	2.07^a ± 0.07	2.45^a ± 0.07	2.53^a ± 0.08
	Basophils	11.37^a ± 0.47	10.78^b ± 0.54	9.88^c ± 0.74	8.02^d ± 0.47	7.89^e ± 0.59	8.33^d ± 0.81
	Eosinophil	11.75^a ± 0.83	10.67^b ± 0.55	10.07^b ± 0.34	8.13^d ± 0.29	9.08^c ± 0.63	9.03^c ± 0.51
	Neutrophils	23.31^a ± 1.88	14.71^c ± 1.62	10.22^d ± 1.33	13.17^cd ± 1.67	17.81^b ± 1.55	22.07^ab ± 1.53
Differential leucocytic counts Parameters	Lymphocytes	55.18^f ± 0.43	63.91^d ± 0.57	72.44^a ± 0.29	69.45^b ± 0.67	66.33^cd ± 0.55	61.11^e ± 0.44
	Monocytes	2.65^a ± 0.04	2.08^a ± 0.04	1.89^b ± 0.03	2.07^a ± 0.07	2.45^a ± 0.07	2.53^a ± 0.08
	Basophils	11.37^a ± 0.47	10.78^b ± 0.54	9.88^c ± 0.74	8.02^d ± 0.47	7.89^e ± 0.59	8.33^d ± 0.81
	Eosinophil	11.75^a ± 0.83	10.67^b ± 0.55	10.07^b ± 0.34	8.13^d ± 0.29	9.08^c ± 0.63	9.03^c ± 0.51
Serum biochemical parameters	TP g/dl	3.24^e ± 0.07	3.33^d ± 0.04	3.93^a ± 0.05	3.82^b ± 0.01	3.65^c ± 0.07	3.32^d ± 0.02
	Alb g/dl	1.91^c ± 0.05	1.89^cd ± 0.02	1.94^b ± 0.02	2.08^a ± 0.01	2.05^a ± 0.05	2.07^a ± 0.02
	Glob g/dl	1.33^e ± 0.06	1.44^d ± 0.02	1.99^a ± 0.03	1.74^b ± 0.04	1.61^c ± 0.04	1.25^f ± 0.02
	Alb/Glob	1.55^b ± 0.03	1.39^c ± 0.02	0.86^e ± 0.03	1.16^d ± 0.02	1.37^c ± 0.03	1.66^a ± 0.03
	S. AST IU/L	23.48^a ± 0.19	21.39^b ± 0.27	18.44^c ± 0.55	19.29^c ± 1.12	17.51^d ± 0.37	21.62^b ± 0.34
	S. ALT IU/L	28.59^a ± 0.44	27.29^ab ± 0.39	21.79^e ± 0.39	23.67^d ± 0.29	24.57^c ± 0.72	25.19^c ± 0.33
Immune status parameters	PA	23.79^f ± 1.42	33.62^e ± 1.47	50.21^a ± 1.84	44.73^b ± 1.24	41.38^c ± 1.61	38.51^d ± 1.34
	PI	3.39^f ± 0.16	6.17^e ± 0.23	9.29^a ± 0.38	8.75^b ± 0.22	7.69^cd ± 0.41	6.88^de ± 0.23
	Lys U/ml	0.22^f ± 0.03	0.26^e ± 0.04	0.39^a ± 0.03	0.36^b ± 0.04	0.34^c ± 0.05	0.33^d ± 0.04
	TI g/dL	1.25^f ± 0.07	1.62^e ± 0.03	2.29^a ± 0.06	1.88^bc ± 0.05	1.83^c ± 0.04	1.65^de ± 0.06

¹D1 (control diet without BAs); diets 2, 3, 4, 5, and 6 were containing commercial BAs 0.10, 0.20, 0.30, 0.40, and 0.50 g/kg diet, respectively.
Means followed by different letters in the same raws differ significantly (P≤ 0.05), n=9.
TP = Total protein, Alb = Albumin, Glob = Globulin, Alb/ Glob = Albumin/ Globulin, AST = aspartate aminotransferase, ALT = alanine aminotransferase, PA = Phagocytic activity, PI = Phagocytic index, Lys = Lysozyme, TI = Total immunoglobulins.

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Table 6 Hematological and biochemical responses of nile tilapia fingerlings fed commercial bile acid supplementation.

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