Table 4 Growth performance of Florida red tilapia (♂ and ♀) fed Moringa oleifera leaf ethanol extract (MOLE) for 60 days pre-spawning and 120 days spawning period, at different salinities.

From: Long term dietary Moringa oleifera leaf extract to Florida red tilapia Oreochromis sp improves performance immunity maturation and reproduction in saltwater

Variable	Salinity	(18ppt)				(32ppt)				P-value
	Diet	D0	D5	D10	D15	D0	D5	D10	D15	Salinity	MOLE	S×M
	Treatment	MSD0	MSD5	MSD10	MSD15	HSD0	HSD5	HSD10	HSD15	Salinity	MOLE	S×M
Female ♀
IBW, g		98 ± 0.15	97.9 ± 0.63	97.3 ± 0.36	97.8 ± 0.34	97.1 ± 0.91	98.4 ± 0.64	96.7 ± 0.34	96.5 ± 0.33	0.393	0.553	0.394
FBW, g		141 ± 0.49^b	140 ± 0.44^b	145 ± 0.81^ab	150 ± 1.71^a	130 ± 0.55^c	139 ± 1.6^b	138 ± 0.78^b	143 ± 2.07^ab	0.483	0.025	0.04
WG, g/fish/day		43 ± 0.53^c	42 ± 0.92^c	47.4 ± 0.69^b	52.4 ± 1.4^a	32.6 ± 1.06^d	40.6 ± 1.06^c	41.6 ± 0.53^c	46.2 ± 1.83^b	0.848	< 0.001	< 0.001
ADG, g/fish/day		0.72 ± 0.01^cd	0.7 ± 0.02^d	0.79 ± 0.01^b	0.87 ± 0.02^a	0.54 ± 0.02^e	0.68 ± 0.02^d	0.69 ± 0.01^d	0.77 ± 0.03^bc	0.848	< 0.001	< 0.001
SGR, %/day/fish		0.61 ± 0.01^b	0.60 ± 0.01^b	0.66 ± 0.01^ab	0.72 ± 0.01^a	0.48 ± 0.02^c	0.65 ± 0.05^ab	0.60 ± 0.01^b	0.652 ± 0.02^ab	0.39	< 0.001	0.001
RGR, %		144 ± 0.57^b	143 ± 1.18^b	149 ± 0.72^ab	154 ± 1.27^a	134 ± 1.36^c	147 ± 4.55^ab	143 ± 0.47^b	148 ± 1.8^ab	0.321	< 0.001	0.002
CF		2.42 ± 0.02^e	3.11 ± 0.11^c	3.38 ± 0.07^ab	3.55 ± 0.00^a	2.61 ± 0.07^e	2.86 ± 0.08^d	3.27 ± 0.13^bc	3.49 ± 0.03^ab	0.612	< 0.001	0.05
Male ♂
IBW, g		123 ± 0.33	123 ± 0.333	123 ± 0.67	123 ± 0.33	123 ± 0.58	123 ± 00	123 ± 0.58	123 ± 0.67	0.811	0.884	0.668
FBW, g		165 ± 0.55^cd	174 ± 0.74^b	175 ± 2.55^b	187 ± 1.68^a	161 ± 0.57^d	168 ± 1.91^c	185 ± 1.79^a	184 ± 1.95^a	0.001	< 0.001	0.007
WG, g/fish/day		42.4 ± 0.3^cd	51.2 ± 0.92^b	51.6 ± 2.11^b	63.8 ± 1.59^a	37.8 ± 0.7^d	45.0 ± 1.91^c	61.8 ± 2.16^a	61.8 ± 2.2^a	0.001	< 0.001	0.014
ADG, g/fish/day		0.71 ± 0.01^cd	0.85 ± 0.02^b	0.68 ± 0.04^b	1.06 ± 0.03^a	0.63 ± 0.01^d	0.75 ± 0.03^c	1.03 ± 0.04^a	1.03 ± 0.04^a	0.001	< 0.001	0.014
SGR, %/day/fish		0.49 ± 0.00^cd	0.58 ± 0.01^b	0.58 ± 0.02^b	0.69 ± 0.01^a	0.45 ± 0.01^d	0.52 ± 0.02^c	0.68 ± 0.02^a	0.68 ± 0.02^a	0.001	< 0.001	0.024
Relative GR, %		134 ± 0.22^cd	142 ± 0.82^b	142 ± 1.57^b	152 ± 1.27^a	131 ± 0.68^d	137 ± 1.55^c	150 ± 1.9^a	150 ± 1.94^a	0.001	< 0.001	0.023
CF		1.56 ± 0.00^e	2.2 ± 0.07^b	2.29 ± 0.04^ab	2.39 ± 0.01^a	1.88 ± 0.02^d	2.04 ± 0.03^c	2.36 ± 0.10^ab	2.27 ± 0.05^ab	0.152	< 0.001	0.001

Means in the same row without a common superscript letter differ significantly (P < 0.05) as analyzed by two-way ANOVA and the TUKEY test. MOLE = Moringa oleifera leaf ethanol extract; S × M = Salinity × MOLE interaction effect; D0 = basal diet without MOLE-supplementation (control); D5 = MOLE-supplemented diet with 5 g/kg; D10 = MOLE-supplemented diet with 10 g/kg; D15 = MOLE-supplemented diet with 15 g/kg; Treatments: MSD0 = fish fed D0 diet @ 18ppt salinity; MSD5 = fish fed D5 diet @ 18ppt salinity; MSD10 = fish fed D10 diet @ 18ppt salinity; MSD15 = fish fed D15 diet @ 18ppt salinity; HSD0 = fish fed D0 diet @ 32 ppt salinity; HSD5 = fish fed D5 diet @ 32 ppt salinity; HSD10 = fish fed D10 diet @ 32 ppt salinity; HSD15 = fish fed D15 diet @ 32 ppt salinity; IBW = initial body weight; FBW = final body weight; WG = weight gain; ADG = average daily gain; SGR = specific growth rate; RGR = Relative growth rate; CF = condition factor.

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Table 4 Growth performance of Florida red tilapia (♂ and ♀) fed Moringa oleifera leaf ethanol extract (MOLE) for 60 days pre-spawning and 120 days spawning period, at different salinities.

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