Fig. 3
LINC01518 regulates the expression of a subset of TGF-β target genes in HNSCC cells. (A) SCC-25 cells transfected with ASO-NS or ASO-1/ASO-2, and transcript levels of indicated genes were measured 48 h post-transfection using qRT–PCR. (B) FaDu cells transfected with ASO-NS or ASO-1/ASO-2, and transcript levels of indicated genes were measured 48 h post-transfection using qRT–PCR. (C) Western blot analysis of Vimentin and E-Cadherin upon LINC01518 knockdown in HNSCC cells. SCC-25 and FaDu cells were transfected with ASO-NS or ASO-1/ASO-2, and protein levels of indicated genes were measured 48 h post-transfection. A representative blot from three independent experiments with similar results is shown. Blots were re-probed for β-actin to establish equivalent loading. (D) Quantification of western blots of indicated proteins shown in (C). (E) Analysis of Vimentin and E-Cadherin expression with or without LINC01518 knockdown during TGF-β treatment of HNSCC cells. Cells were transfected with ASO-NS or ASO-1, and twenty-four hours after transfection, treated with or without TGF-β (10 ng/ml for 48 h), and protein levels of indicated genes were measured. A representative blot is shown from three independent experiments with similar results. Blots were re-probed for β-actin to establish equivalent loading. (F) Quantification of western blots of indicated proteins shown in (E). Data information: (For A–F) Error bars represent the mean ± SEM from three independent experiments. (For A–D) *Significant change compared to ASO-NS (p < 0.05). (For F) **Significant change compared to Control+ASO-NS cells (p < 0.05). #Significant change compared to TGF-β+ASO-NS cells (p < 0.05). *Significant change compared to Control+ASO-NS cells (p < 0.05). Statistical comparisons were made using the Student’s t-test.
