Fig. 4
From: In vitro antiviral activities of thymol and Limonin against influenza a viruses and SARS-CoV-2
Biochemical assays to explore the anti-polymerase and anti-neuraminidase activities of Limonin and Thymol. (a) Minigenome assay to validate possible biological interaction of Thymol and Limonin with influenza A/H1N1 polymerase subunits as determined by ZsGreen expression in treated and untreated transfected HEK 293T cells or (b) by measuring the nanoluciferase activity in the supernatants of treated and untreated transfected HEK 293T cells. (c) The neuraminidase inhibition assay against influenza A/H1N1 showed that Limonin and Thymol have a rare effect on viral neuraminidase activity.
