Fig. 4
From: In vivo efficacy of NRL knockdown with cell-penetrating siRNA in retinal degeneration
cp-asiNRL knockdown efficacy in RhoP23H/+ transgenic mice. (A) Representative western blot images. (B) Relative densitometric bar graphs (n = 4 per group). (C) Immunohistochemistry images showing cone- and rod-specific markers in the dorsal retina. (D) Quantification of the number of cells displaying positive fluorescence for S-opsin and L/M-opsin in control and cp-asiNRL-treated retinas. Data are presented as mean ± SD (n = 3 per group). (E) Representative images of apoptosis in the outer retinal layer were detected using a TUNEL assay. The blue color denotes all nuclei, and the green color denotes DNA-damaged nuclei. The graph shows a significantly decreased ratio of apoptotic nuclei in the cp-asiNRL-treated group compared to the control group. (F) Electroretinogram under scotopic and photopic conditions (n = 5 per group). *p < 0.05.
