Fig. 1

Comparison of gene expression profiles between Monolayer cells derived from healthy Human Colonic Organoid (MHCO) and Caco-2. (A) A schematic representation of the experiment. (B) PCA of the transcriptomes of MHCO and Caco-2. Transcriptomes from three replicates were plotted. Ellipses indicate the 95% confidence interval. Filled and unfilled circles indicate two independent experiments (each three biological replicates). (C) Volcano plot depicting the DEGs of the transcriptomes of MHCO and Caco-2. The x- and the y-axis show the log₂ transformed fold change and the -log₁₀ transformed adjusted p-value. Red and blue dots indicate significantly upregulated genes in MHCO and Caco-2 according to adjusted p-value < 0.01 and log₂ fold change > 1.5. (D) Heatmap depicting the top-1000 DEGs of the transcriptomes of MHCO and Caco-2 when sorted by adjusted p-value. (E) TPM values of marker genes for each gut epithelial cell type. Data are presented as the mean ± Standard derivatives (SD). Statistical significance was evaluated by Deseq2.