Fig. 7 | Scientific Reports

Fig. 7

From: Comprehensive gene expression analysis of organoid-derived healthy human colonic epithelium and cancer cell line stimulated with live probiotic bacteria

Fig. 7

B. longum regulates intestinal barrier function. (A) Heatmap of relative expression of cell junction-related genes by co-cultivation of B. longum in Caco-2 and MHCO. Statistical significance was evaluated by a DESeq2 (*adjusted p-value < 0.05, ** adjusted p-value < 0.01, *** adjusted p-value < 0.001). (B–E) TPM values of representative tight junction (B), adherence junction (C), desmosome (D), and hemidesmosome (E) related genes. Filled and unfilled circles indicate two independent experiments (each three replicates). Data are presented as the mean ± Standard derivatives (SD). Statistical significance was evaluated using a Deseq2. (F, G) Relative TEER value adjusted by the initial value (F) and permeability of Lucifer Yellow (LY) from apical to basolateral compartment (G) in MHCO, and relative TEER value adjusted by the initial value in Caco-2 (H) cocultured with B. longum versus vehicle control. Data are expressed as means ± SD. Statistical significance was evaluated using Welch’s t-test.

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