Fig. 4

The results of Barleria extracts, barlerin and verbascoside activate PBMCs against PC-3 implicated to cytokine genes expression. (A) a schematic of activated PBMCs establishing and cytotoxic testing procedure using MTT assay; (B) a heatmap representing percentage of cell viability obtained after 24 and 48 h co‐culture; n = 3; (C) the genotoxic result showing comet image at 200x to reveal DNA damage of PC‐3 cell in each condition after 48 h co‐culture; n = 150; (D) The dot plot of single cell to indicate apoptotic PC‐3 cell and comparing bar chart of the percentage of apoptosis in each condition after 48 h co‐culture; n = 3; (E) the histogram of PC‐3 cell cycle after 48 h co‐culture showing DNA content in peak in subG₁, G₁, S, and G₂‐M phases and comparing bar chart of percentage DNA content; n = 3; (F) the bubble plot to show percentage relative cytokine gene expression in PBMCs normalized by β‐actin as a reference gene; n = 3; (G) the procedure of PBMCs sorting using Flow cytometric assay; (H) The population of CD3⁺CD56⁻ T cell, CD4⁺ T cell, CD8⁺ T cell and CD56⁺CD3⁻ NK cell in studied PBMCs after 48 h treatment; n = 3.