Fig. 1

In silico analysis of putative GABA permease (CsgabP, aka amino-acid permease [BAT1]) from Citrus sinensis. Evolutionary analysis using the maximum likelihood method and its associated multiple protein sequence alignments using Constraint-Based Alignment tool (COBALT) analysis. The listed genes were identified using the protein-protein BLAST (BLASTp) using bidirectional amino acid transporter 1 (BAT1; GenBank accession no. NP_565254.1; 516 aa) from Arabidopsis thaliana (aka GABA permease [AtgabP])¹⁶ as a query sequence against Citrus sinensis genome available in GenBank, National Center for Biotechnology Information website (NCBI, http://www.ncbi.nlm.nih.gov/gene/), using the compositionally adjusted substitution matrices³⁵. Evolutionary analyses and the joint tree were conducted in MEGA-X software³⁶. In the COBALT analysis, residues were colored using a column-based method according to their relative entropy threshold. Aligned columns with no gaps are colored blue and red, where the red color indicates highly conserved columns and blue indicates less conserved ones. The full list of genes, names, and accession numbers is available in supplementary Tables S2 and S3.