Fig. 2

Evolutionary relationships of GABA permease (gabP, aka amino-acid permease [BAT1]) proteins from different plant species. The evolutionary history was inferred by using the Maximum Likelihood method and JTT matrix-based model³⁷. The tree with the highest log likelihood (-65227.88) is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches³⁸. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the JTT model and then selecting the topology with a superior log-likelihood value. This analysis involved 106 amino acid sequences. There were a total of 1052 positions in the final dataset. Evolutionary analyses were conducted in MEGA X³⁶. Putative GABA permeases of interest from Citrus sinensis (CsgabP) are bolded and marked by a black circle. The listed genes were assembled based on recently available data in GenBank, National Center for Biotechnology Information website (NCBI, https://www.ncbi.nlm.nih.gov/protein/). The full list of genes, names, and accession numbers is available in supplementary Table S5.