Fig. 3
In silico analysis of putative GABA permeases (CsgabP, aka amino-acid permease [BAT1]) from Citrus sinensis. (A) Multiple sequence alignment of the top matched AA sequence of putative CsgabPs from C. sinensis along with the query sequence of bidirectional amino acid transporter 1 (BAT1; GenBank accession no. NP_565254.1; 516 aa) from Arabidopsis thaliana (aka GABA permease [AtgabP])16. Clustal Omega algorithm39 and QIAGEN CLC Genomics Workbench 24.0.2 were used for the multiple sequence alignment and visualization of retrieved AA sequences. Conserved amino acids and similar residues are shaded using RasMol coloring. (B) Novel motifs of CsgabP-1 (XP_006468761.1) and CsgabP-2 (XP_006468762.1) from sweet orange (C. sinensis), along with the query protein sequence, AtgabP (NP_565254.1). Motifs were discovered using Multiple Em for Motif Elicitation (MEME) Suite-version 5.5.7 (http://meme.sdsc.edu/meme/meme.html, accessed on October, 2nd, 2024)40. (C and D) Functional and conserved domains analysis of CsgabP-1 (XP_006468761.1) and CsgabP-2 (XP_006468762.1), respectively, using the InterPro Scan tool. Family and conserved sites corresponding to the entry of three contributing databases (InterPro, PANTHER, and PIRSF) as follows; IPR002293: amino acid/polyamine transporter I family, IPR004756: amino acid permease subfamily, IPR004840: amino acid permease conserved site, PTHR45649:SF48: amino-acid permease BAT1 homolog, PTHR45649: amino-acid permease BAT1, G3DSA:1.20.1740.10: amino acid/polyamine transporter I, and PIRSF006060: amino acid transporter. The full list of genes, names, and accession numbers is available in supplementary Table S2.
