Table 2 Primers used for qRT-PCR analysis.
Gene | Source of isolation | Gene bank accession number | Nucleotide sequence of primers (5′–3′) | Annealing temp. (°C) | Size (bp) |
|---|---|---|---|---|---|
IL-6 | Spleen | XM_019350387.2 | F: AAACCAATTCCTTCTGGCCCT R: TCACCTGAGAAGTCACCTGC | 56 | 189 |
IL-8 | Spleen | NM_001279704 | F: CTGTGAAGGCATGGGTGTG R: ATCACTTTCTTCACCCAGGG | 58 | 196 |
CAT | Liver | JF801726.1 | F: TTTGCACGTTTACAGCCGTC R: CTGATGGCTCGAATAATGGTCC | 60 | 129 |
SOD | Liver | JF801727.1 | F: ACGTGACAACACAGGTTGCT R: AGTCCCGTTTGATTGCCTCC | 58 | 144 |
GH | Liver | XM_003442542 | F: TAATGGGAGAGGGA AGATGG R: CTCTGCGATGTAATTCAGGA | 60 | 202 |
IGF-I | Liver | XM_003448059 | F: TTGTCTGTGGAGAGCGAGGCTT R: CAGCTTTGGAAGCAGCACTCGT | 62 | 90 |
PGAM2 | Muscle | XM_003444358.5 | F: GCTATCAAGGAGGCAGGCAT R: GACGCCAGGTACGAATCACA | 58 | 130 |
β-actin | Spleen, liver, and muscle | XM_003443127 | F: GTGCCCATCTACGAGGGTTA R: CTCCTTAATGTCACGCACGA | 60 | 156 |
