Fig. 4
From: Macrophage induces angiogenesis via HIF signaling in denervated muscle following nerve injury
Macrophages Sensing Hypoxia in Denervated Muscle Activates HIF Signaling. (A) Representative images showing Hypoxiprobe-1 distribution in ipsilateral lumbrical muscle at PO days 3 and 28. The graph quantifies the number of Hypoxiprobe-1-positive cells per 1000 × 1000 μm. ****P<0.001, ***P<0.005, *P<0.05, by one-way ANOVA with Dunnett’s multiple-comparison test. (B) mRNA expression levels of Hif1-α, Vegf, and Glut1 in contralateral and ipsilateral lumbrical muscles at PO days 3 and 28. ****P<0.001, ***P<0.005, **P<0.01, by one-way ANOVA with Dunnett’s multiple-comparison test. (C) Representative images of immunostaining for CD68 in contralateral and ipsilateral TA muscles at PO day 3. (D) Representative images showing double immunostaining for CD68 (red) and Hypoxiprobe-1 (green) in ipsilateral TA muscles at PO day 3. The pie chart shows the cell composition of Hypoxiprobe-1-positive cells in ipsilateral TA muscle. (E) Representative immunostaining for CD68 in PLX3397-treated TA muscle. The left image shows the contralateral nerve treated with PLX 3397, the middle image shows the ipsilateral nerve at PO day 3, and the right image shows the ipsilateral nerve treated with PLX 3397 at PO day 3. The graph quantifies the number of CD68-positive cells per 1000 × 1000 μm. ***P<0.005, **P<0.01, by one-way ANOVA with Dunnett’s multiple-comparison test. (F) mRNA expression levels of Hif1-α, Vegf, and Glut1 in PLX3397-treated contralateral and ipsilateral lumbrical muscles at PO day 3. ****P<0.001, ***P<0.005, **P<0.01, by one-way ANOVA with Dunnett’s multiple-comparison test.
