Fig. 3
From: miR-210 locus deletion disrupts cellular homeostasis: an integrated genetic study
Transcriptomic consequences of locus disruption in mouse and human cells. Pathway enrichment analysis of gene expression in miR-210-KO mouse fibroblasts and HEK cells under low O2 (1%) was performed using Enrichr and visualized with an Appyter for bulk RNA-seq (https://appyters.maayanlab.cloud/#/Bulk_RNA_seq). (a) Scatter plot with larger, black-outlined points highlighting significantly enriched pathways, grouped by gene set similarity. (b) Bar charts displaying the most significant gene sets from MSigDB_Hallmark_2020, with levels of significance indicated. (c) Volcano plots showing microRNAs potentially affecting downregulated DEGs in mir210-KO cells, with each microRNA represented by its odds ratio and statistical significance. (d–f) Similar analysis for MIR210-KO HEK cells under 1% O2, applying a stricter significance threshold (p-adjusted < 0.01) to limit gene number for more precise enrichment analysis. (g–i) Parallel analysis in MIR210HG-KO HEK cells under similar experimental setups, detailing transcriptomic shifts and pathway dynamics. For all analyses, DEGs were defined based on a log₂ fold-change cutoff of 0.3.
