Fig. 2
Myeloid cell commitment from CD34 + HSPCs in AMO. (A) Morphological evaluation of one representative myeloid cell differentiation at 21 days observed after MGG staining of AMO cell cytospin. One representative experiment out of three is presented. Black arrow: immature myeloid cells. Red arrow: mature myeloid cells. Scale bar 60 μm (B-C) Analysis at day 7 (left) and 21 (right) of a representative whole mount organoid stained with Hoechst (nuclei, blue) and CD45-AF647 (hematopoietic counterpart cell membrane, red). (B) Picture of one representative experiment out of three is presented. Scale bar 100 μm. (C) Quantification of the CD45 intensity (N = 15808 and 11237 cells, at day 7 and day 21 respectively) from 3 different experiments; p-value of paired t test < 0.001. Data are represented as mean ± SD. (D) Spatial cells´ distance of CD45 + cells to the organoid border (black) or center (red) at day 7 for 4 independent AMO numerated from 1 to 4 (N = 1545, 11606, 14282, 2421 cells analyzed for independent organoid). Data are represented as mean ± SD.
