Fig. 2

The T-cell magnitude is significantly higher in SARS-CoV-2 seropositive samples than in seronegative samples. SI (a) and SII (b) magnitude is significantly higher for both adults (blue) and children (orange) in SARS-CoV-2 seropositive samples than in seronegative samples. As a control, pre-pandemic samples (n=31) are also included. Scattered dot plots with the line representing the mean. CO value for positivity in the cellular assay is indicated by the dashed line. 0 values were adjusted to 0.01 for display purposes only; all analysis was performed using unadjusted data. Statistical significance was determined by one-way ANOVA (Kruskal-Wallis) with Dunn’s multiple comparison test, with * indicating a p0.05, **** indicating a p0.0001 and ns indicating a p0.05. To evaluate whether the magnitude of T cell response was correlated with titer, SI/SII magnitudes were compared to titers of different SARS-CoV-2 antigens in combined individuals (adults n=99, children n=28). Combined individuals were used to avoid bias introduced by negative values. Due to the overlap between the peptide pools used for stimulation and the nucleocapsid antigen, the correlations for the nucleocapsid-specific antibodies are shown here (c and d). Correlation analysis for the other SARS-CoV-2 antigens can be found in Extended Data Table 4 and Extended Data Figure 2. Dot plots showing correlation between SI (c) and SII (d) magnitude against nucleocapsid-specific antibody titer. Linear regression with Pearson r is used to determine the strength of correlation, with Pearson r, r2 values and p-values provided.  Adults are shown in blue, children in orange.