Fig. 8

In silico expression levels of WAKL4 and VIT PPI networks in Zn deficiency and Zn homeostasis-related mutants. Expression levels of WAKL4 and VIT protein networks under zinc deficiency were analyzed in the roots and shoots of wild-type Col-0 after 7 days (GSE77286) and 21 days (GSE111443) in hydroponic conditions. Expression patterns in bzip19bzip23 and hma2hma4 double mutant roots and shoots were also evaluated. A heat map illustrating these expression patterns was generated using the “plotty” function in the ggplot2 package in R, utilizing Euclidean distance hierarchical clustering.