Fig. 2 | Scientific Reports

Fig. 2

From: Circular RNA circFat3 as a biomarker for construction of postmortem interval Estimation models in mouse brain tissues at multiple temperatures

Fig. 2

Validation of circFat3 in mouse brain tissue. (A) The analysis using convergent and divergent primers of circFat3 in cDNA and gDNA samples. For circFat3, the product lengths of divergent and convergent primers were 276 bp and 284 bp, respectively. (B) The RNase R digestion analysis of circFat3, linear Fat3, and Gapdh in ddH2O group, 0 U group and 1 U group. (C) The data analysis of the relative expression of circFat3, linear Fat3, and Gapdh after the RNase R digestion. (D) The structure of circFat3 and the results of Sanger sequencing.

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