Fig. 7

Construction and validation of 35 °C PMI models using RT-qPCR. (A) The melting curves of circFat3. (B) The melting curves of mt-co1. (C) The amplification curves of circFat3. (D) The amplification curves of mt-co1. (E) The linear, quadratic, and cubic equations constructed based on the ΔCt values of circFat3 and mt-co1 at 35 °C. h, hours.