Table 1 Establishment of the mathematical models for 4 °C PMI estimation by semi-quantitative RT-PCR.

From: Circular RNA circFat3 as a biomarker for construction of postmortem interval Estimation models in mouse brain tissues at multiple temperatures

Temperature

circRNA

Reference gene

Equation

 

R2

P

4 °C

circFat3

mt-co1

Linear

Y = -0.04098X + 0.8063

0.8604

0.0026

Quadratic

Y = 0.8749–0.08176X + 0.002626X2

0.9208

0.0063

Cubic

Y = 0.9429–0.1633X + 0.01646X2-0.0005742X3

0.9724

0.0077

28S rRNA

Linear

Y = -0.04774X + 1.017

0.9900

0.0000

Quadratic

Y = 1.025–0.05209X + 0.0002798X2

0.9906

0.0001

Cubic

Y = 0.9955–0.01731X-0.005624X2 + 0.0002451X3

0.9986

0.0001

mt-co1/28S rRNA

Linear

Y = -0.04436X + 0.9118

0.9705

0.0001

Quadratic

Y = 0.9497–0.06693X + 0.001453X2

0.9883

0.0001

Cubic

Y = 0.9692–0.09028X + 0.005417X2-0.0001646X3

0.9924

0.0011

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