Table 2 Verification of the 4 °C mathematical models by semi-quantitative RT-PCR.
Temperature | circRNAs | Reference gene | Real PMI | Estimation PMI | Estimation Error | Error rate (%) | Equation |
|---|---|---|---|---|---|---|---|
4 °C | circFat3 | mt-co1 | 2d | 6.5100 | 4.5100 | 225.50% | Linear |
2d | 4.8609 | 2.8609 | 143.05% | Quadratic | |||
2d | 3.6304 | 1.6304 | 81.52% | Cubic | |||
6.25d | 7.1388 | 0.8888 | 14.22% | Linear | |||
6.25d | 5.3294 | 0.9206 | 14.73% | Quadratic | |||
6.25d | 4.0436 | 2.2064 | 35.30% | Cubic | |||
9.25d | 8.9496 | 0.3004 | 3.25% | Linear | |||
9.25d | 6.8177 | 2.4323 | 26.30% | Quadratic | |||
9.25d | 5.7316 | 3.5184 | 38.04% | Cubic | |||
28S rRNA | 2d | 4.5425 | 2.5425 | 127.12% | Linear | ||
2d | 4.4217 | 2.4217 | 121.09% | Quadratic | |||
2d | 4.9791 | 2.9791 | 148.95% | Cubic | |||
6.25d | 6.3275 | 0.0775 | 1.24% | Linear | |||
6.25d | 6.1563 | 0.0937 | 1.50% | Quadratic | |||
6.25d | 6.4622 | 0.2122 | 3.40% | Cubic | |||
9.25d | 5.9675 | 3.2825 | 35.49% | Linear | |||
9.25d | 5.8037 | 3.4463 | 37.26% | Quadratic | |||
9.25d | 6.1710 | 3.0790 | 33.29% | Cubic | |||
mt-co1/28S rRNA | 2d | 7.5351 | 5.5351 | 276.75% | Linear | ||
2d | 6.4697 | 4.4697 | 223.49% | Quadratic | |||
2d | 6.2229 | 4.2229 | 211.15% | Cubic | |||
6.25d | 8.0597 | 1.8097 | 28.96% | Linear | |||
6.25d | 6.9590 | 0.7090 | 11.34% | Quadratic | |||
6.25d | 6.7930 | 0.5430 | 8.69% | Cubic | |||
9.25d | 8.3524 | 0.8976 | 9.70% | Linear | |||
9.25d | 7.2398 | 2.0102 | 21.73% | Quadratic | |||
9.25d | 7.1280 | 2.1220 | 22.94% | Cubic |
