Table 8 Verification of the 35 °C mathematical models by qPCR.

From: Circular RNA circFat3 as a biomarker for construction of postmortem interval Estimation models in mouse brain tissues at multiple temperatures

Temperature

circRNAs

Reference gene

Real PMI

Estimation PMI

Estimation Error

Error rate (%)

Equation

35 °C

circFat3

mt-co1

24 h

48.02

24.02

100.10%

Linear

   

24 h

36.48

12.48

51.99%

Quadratic

   

24 h

37.94

13.94

58.08%

Cubic

   

30 h

46.07

16.07

53.56%

Linear

   

30 h

34.93

4.93

16.43%

Quadratic

   

30 h

36.55

6.55

21.82%

Cubic

   

60 h

79.21

19.21

32.02%

Linear

   

60 h

73.77

13.77

22.95%

Quadratic

   

60 h

66.67

6.67

11.11%

Cubic

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