Fig. 3 | Scientific Reports

Fig. 3

From: Extraction of lichen bioactive compounds using volatile natural deep eutectic solvents and comparative analytical approaches

Fig. 3

Effect of extraction method: ultrasound-assisted extraction (UAE), maceration (MAC), and accelerated solvent extraction (ASE), and solvent type: acetone, ethyl acetate on extraction yield (bars) and total lichen acid content (dots) in successive stages of the extraction sequence (I-V) and for the total amount collected from all extraction steps. Data are mean ± SD (n = 3). Different letters indicate significant differences between the samples, calculated based on a 2-way ANOVA and post-hoc Tukey analysis Two-way ANOVA results: p-values indicate the effects of factors: A (extraction method), B (solvent type), and AxB (interaction between method and solvent). Data are presented as means (n = 3) ± standard deviation (SD).

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