Table 2 Predicted values and experimental mean values (mg/g DW) at the optimal extraction conditions for X1: menthol/camphor ratio (1.5:1); X2: liquid/solid ratio (100:1, volume/mass); X3: temperature of extraction (40 °C); X4: time of extraction (30 min).

From: Extraction of lichen bioactive compounds using volatile natural deep eutectic solvents and comparative analytical approaches

Response variables

Predicted mean

Observed mean value

Std dev

RD (%)

95% PI low

95% PI high

Physodalic acid (mg/g DW)

38.05

32.28

5.163

17.875

30.570

45.529

3-Hydroxyphysodic acid (mg/g DW)

39.60

35.02

5.049

13.078

31.990

47.200

Physodic acid (mg/g DW)

27.52

29.96

3.575

− 8.144

22.361

32.670

Atranorin (mg/g DW)

11.28

12.48

1.783

− 9.615

8.689

13.871

Chloroatranorin (mg/g DW)

3.50

3.09

0.572

13.269

2.683

4.3105

Cumulative lichen acid (mg/g DW)

117.29

112.82

13.517

3.962

97.799

136.779

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