Fig. 3
Cell proliferation dynamics of MSCs ‒measured as resofurin fluorescence intensity (Ex. 550/Em. 590 nm; mean ± SE) were analyzed to assess the growth of MSCs. MSCs cultured on different substrates (ME, LAR, HAR, hierarchies (LAR + ME)) made in three different materials: (a) sPDMS, (b) hPDMS, and (c) PMMA. (d) MSCs cultured on flat sPDMS, hPDMS, and PMMA polymers. One-way ANOVA test followed by Holm-Šídák post-hoc analysis was carried out for comparing the MSCs proliferation rates between polymers at each time point. Significant differences are marked by */# (p < 0.05) for both sPDMS and hPDMS respectively. PS flat substrate from tissue-culture plates was included as a control. Gray shading highlights the top-performing conditions, emphasizing the early-culture response (days 4–5), where the highest growth increment occurs relative to the PS control (threshold reference). Gray arrows denote the phase of maximal proliferation increase.
