Fig. 5
One-way ANOVA analysis followed by Holm-Šídák post-hoc test comparing the length of the mean resultant vector, R , which represents the directionality degree acquired by MSCs cultured on the different substrates. Significant data is established by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001) and non-significant (ns) for the corresponding comparisons. (a) Representative fluorescence images of cells grown in the three main geometric arrangements analyzed (Gratings, SPA, HPA) ‒exemplified by the PMMA substrate‒ and their respective rose diagrams below show their angular distribution (violet facets) and their main acquired orientation (R values shown by the red line). (b) The plot shows a comparison between the flat PS control and the different substrates studied. The high R values (R >0.6) are noteworthy for the most anisotropic topographies, whichexhibited particularly significant differences.
