Fig. 1

The effect of various fluorescence spectra on Raman sampling. (a) Sodium fluorescein (NaFl): excitation/emission \(\:\approx\:\)498 nm (dark green)/\(\:\approx\:\:\)517 nm (light green) and its Raman spectrum in a HEK293 cell (solid line) with the first standard deviation (shade). (b) eGFP: excitation/emission 488 nm (dark green)/507 nm (ocher) and its Raman spectrum in a HEK293 cell (solid line) with the first standard deviation (shade). (c) mTagBFP2: excitation/emission 405 nm (dark blue)/454 nm (light blue) and the Raman spectrum of mTagBFP2-Cx43 in a HEK293 cell (solid line) with the first standard deviation (shade). (a-c) Raman spectra are not baselined. Spectra were obtained from FPbase³⁵ (d-f) HEK293 cells expressing mTagBFP2-Cx43. White bar \(\:\approx\:\:\)5 μm (d) Fluorescence imaging before (left) and after (right) Raman sampling on a fluorescent microscope shows no significant loss in fluorescence. The sites for Raman sampling can be identified in correlating brightfield imaging on the Raman microscope (middle).